St. Paul, Minn. (March 8, 2010) – AACC International Approved Method 32-45.01, Total Dietary Fiber (Codex Alimentarius Definition), was approved after rigorous review by the AACC Intl. Approved Methods technical Committee. This new method is important because it simplifies TDF analysis when several types of fiber are formulated in foods. It is now available online through AACC Intl. Approved Methods of Analysis, 11th Edition.
AACC Intl. Method 32-45.01 determines total dietary fiber in foods and food ingredients, as defined by the Codex Alimentarius Commission. The method quantitates high molecular weight dietary fiber (HMWDF), including resistant starch (RS), and low molecular weight soluble dietary fiber (LMWSDF), including nondigestible oligo-saccharides. In this method, enzymatic digestion at 37°C is used to simulate human intestinal digestion, followed by gravimetric isolation to quantitate HMWDF and high-performance liquid chromatography to quantitate LMWSDF. The method measures the complete range of dietary fiber components from RS to digestion-resistant oligosaccharides.
The collab for this new method consisted of 18 laboratories. The examination of the intralaboratory results was completed by the AACC Intl. Dietary Fiber Committee and the Approved Methods Technical Committee. A video demonstration and online calculators for this new fiber method will be added soon upon approval by the Approved Methods Enhancements Review Committee. However, the method is valid and ready for use in food labs.
AACC International Approved Methods of Analysis, 11th Edition online subscribers will receive free access to this new method. To request a subscription quote, go to the Approved Methods of Analysis, 11th Edition website. This method may also be purchased as an individual PDF file for $200. To purchase this single method call toll-free 1.800.328.7560 U.S.A. and Canada or +1.651.454.0766 elsewhere.