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Cereal Chem 62:19-22   |  VIEW ARTICLE
Effect of Temperature and Alternate Lactate Buffer Systems on Resolution of Wheat Gliadin Proteins by Polyacrylamide Gel Electrophoresis.

G. L. Lookhart, D. B. Cooper, and B. L. Jones. Copyright 1985 by the American Association of Cereal Chemists, Inc. 

Polyacrylamide gel electrophoregrams of wheat gliadins at different constant gel temperatures and in sodium and aluminum lactate buffer systems were compared in terms of band resolution, band curvature, and electrophoresis time. Electrophoresis of gliadins was improved in both the sodium and aluminum lactate systems by reducing the temperature from 21 C to 7 or 10 C. No major differences in resolution or time were found between the two systems at constant voltage (290 v) and 10 C. Minor differences were noted, whereby one gel system gave better resolution on particular proteins but the other system resolved other proteins better.

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