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Detection of Irradiated Wheat Using the Electron Paramagnetic Resonance Spin Probe Technique

¹Department of Physics, Zonguldak Karaelmas University, 67100, Zonguldak, Turkey. ²Corresponding author. E-mail: dadayli@elmas.bim.karaelmas.edu.tr Fax: 01090 03722572070. ³Department of Physics Engineering, Hacettepe University, 06532, Beytepe, Ankara, Turkey. ⁴Department of Food Engineering, Hacettepe University, 06532, Beytepe, Ankara, Turkey.

In this study, the electron paramagnetic resonance (EPR) spin probe technique is applied as a detection method in the differentiation of irradiated and unirradiated wheat seeds. Two wheat cultivars, Kunduru and Bezostaya, were used. Aqueous solutions of 4-hydroxy-TEMPO (TANOL) spin probe prepared with a line-broadening agent, potassium ferricyanide, were used in all experiments. The EPR spectra of the samples were recorded against time. A decrease in the signal intensity and a change in the shape of the intensity-time curve (rehydration curve) were observed, depending on the applied level of irradiation. The ratio of the lipid and aqueous regions at the high field (m_I = -1) line changes, depending on the dose of irradiation.