  2001 AACC Annual Meeting Charlotte, North Carolina October 14-18, 2001 Charlotte Convention Center  |
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Characterization of water insoluble components of dietary fiber in oats and barley. L. JOHANSSON (1), L. Virkki (1), M. Ylinen (1), P. Ekholm (1), and H. Salovaara (2). (1) Department of Applied Chemistry and Microbiology, University of Helsinki, FIN-00014, Helsinki, Finland; (2) Department of Food Technology, University of Helsinki, FIN-00014, Helsinki, Finland.
The aim of the work was to study the components of the insoluble fiber of oats and barley which have not been studied in detail with specific interest on beta-glucan and the fraction that remains after alkaline extraction. Comparing the structures of soluble and insoluble beta-glucan will elucidate the structure-properties-relationship for this polymer which is related to the health effects of oats. Water insoluble dietary fiber of oats and barley was isolated (Asp et al.) and further sequentially extracted by the modified methods of Gruppen et al. and Nilsson et al.. The extractants used were barium hydroxide, water, 1 M potassium hydroxide and 4 M potassium hydroxide. The extracted polymers were characterized by measuring their neutral sugar composition using gas chromatography. Further characterization of fractions was made by (^1)H NMR and (^13)C NMR measurements and by enzymatic digestion followed by capillary electrophoresis. These methods were used in our earlier studies of water soluble dietary fiber components (Johansson, L. et al.)
Copyright 2001
The American Association of Cereal Chemists