  2001 AACC Annual Meeting Charlotte, North Carolina October 14-18, 2001 Charlotte Convention Center  |
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Relation of viscoelastic properties and capillary zone electrophoresis mapping of high molecular weight-glutenins subunits fractions obtained with RP-HPLC. G. DAVILA-HUERTA (1), J. Mitchum (1), P. Rayas-Duarte (1), and Z. El-Razzi (2). (1) Food and Agricultural Products Research Center; (2) Department of Chemistry, Oklahoma State University.
Viscoelastic properties of HMW-GS fractions and their CZE maps can be compared to reveal the magnitude of the contribution of specific subunits. The objective of this study was to compare the elastic and viscous components of HMW-GS fractions obtained from RP-HPLC with their CZE mapping. HMW-GS were extracted from two hard red winter wheats, cv. Jagger and Big Dawg, of contrasting baking properties, using 50% n-propanol containing 1% DTT. Extracts were loaded onto a RP-HPLC equipped with a Hi-Pore304 column and photodiode array detector. Fractions were separated with a linear gradient from 25 to 50% acetonitrile. The RP-HPLC profile of the two cultivars were subtracted and the resulting peaks identified. A second subfraction containing the different peaks was collected using preparative RP-HPLC and their viscous and elastic components analyzed using a control stress rheometer. CZE mapping of this subfraction was correlated with its viscous and elastic moduli, and tan delta(d). Quantitative viscoeleastic properties of this subfraction partially explains the differences of the two cultivars.
Copyright 2001
The American Association of Cereal Chemists