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NOVEMBER
5-9, 2000 KANSAS CITY, MISSOURI
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A A C
C 2 0 0 0 A n n u a l M e e
t i n g
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54
Gluten protein synthesis and glutenin polymer assembly in grain of developing wheat. R. Wright (1),
A. S. Tatham (1), K. Feeney (1), F. Bekes (2), N. Wellner (3), and P. R. SHEWRY (1). (1) IACR-Long
Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK; (2) Plant Science CIC, CSIRO, North
Ryde, Australia; (3) Institute of Food Research, Norwich, NR4 7UA, UK.
The time course of synthesis of gluten proteins has been studied in
developing endosperms of spring wheat cv. Axona. Glutenin polymers have also been extracted and
separated by size exclusion HPLC and Field Flow Fractionation. Protein bodies have been prepared from
various developmental stages and analysed to determine their compositions of glutenin polymers and their
conformation using FT-IR spectroscopy. Preliminary studies have also been carried out on protein bodies
and protein fractions from grain of winter wheat grown under conditions of low water availability and heat
stress. The results are providing new information on the assembly of glutenin polymers in the developing
grain and how this is affected by growth conditions.
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