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NOVEMBER
5-9, 2000 KANSAS CITY, MISSOURI
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A A C
C 2 0 0 0 A n n u a l M e e
t i n g
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38
Molecular wheat protein quality via a dedicated near-IR delta dichroic ratio polymer orientation stretching
response gauge. DAVID L. WETZEL. Department of Grain Science and Industry, Kansas State
University.
Delta dichroic ratio data are presented from application of the
automated custom built gauge to gluten films from a group of experimental (and released) hard winter
wheat varieties. The procedure takes place using a dedicated near-IR instrument that compares dichroic
ratios of select absorption bands of the polyamide gluten before and after stretching to a predetermined
elongation. Automated elongation of the mounted specimen and rotation of the specimen in the polarized
beam of an acousto-optic tunable filter random wavelength access spectrometer minimized operator effect
on the results. The gluten film is cast from a solution obtained by a 3 step process starting with flour of
individual wheat varieties. Unstretched film is placed on the jaws of the sample holder, a microprocessor
controlled stepping motor provides programmable metered elongation and the specimen rotation (in the
polarized near-IR beam) is pneumatically activated. The dedicated spectrometer is intended for routine use
to support the wheat breeding program of the Kansas Agricultural Experiment Station. The same computer
that controls the gluten film stretching and sample rotation also performs electronically switched random
wavelength access by programmed input to an accousto-optic tunable filter. Electronic wavelength
switching also allows the versatility of scanning the entire spectrum.
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