Publication no. C-2003-1216-01R |  VIEW ARTICLE

REVIEW: Mixed Linkage (1-3),(1-4)-beta-D-Glucans of Grasses.

Marcos S. Buckeridge (1), Catherine Rayon (2,3), Breeanna Urbanowicz (2,4), Marco Aurélio S. Tiné (1), and Nicholas C. Carpita (2,5). (1) Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica CP 4005 CEP 01061-970, São Paulo, SP Brazil. (2) Department of Botany and Plant Pathology, Purdue University West Lafayette, IN 47907-1155. (3) Present address: UMR CNRS-UPS 5546, Pôle de Biotechnologie Végétale, BP 17, Auzeville, F-31326 Castanet Tolosan, France. (4) Present address: Department of Plant Biology, 228 Plant Science Building, Cornell University, Ithaca, NY 14853. (5) Corresponding author. Phone: +1-765-494-4653. Fax: +1-765-494-0393. E-mail: <carpita@purdue.edu> Cereal Chem. 81(1):115-127. Accepted June 24, 2003. Copyright 2004 American Association of Cereal Chemists, Inc.

The mixed-linkage (1-3),(1-4)-beta-D-glucans are unique to the Poales, the taxonomic order that includes the cereal grasses. (1-3), (1-4)-beta-Glucans are the principal molecules associated with cellulose microfibrils during cell growth, and they are enzymatically hydrolyzed to a large extent once growth has ceased. They appear again during the developmental of the endosperm cell wall and maternal tissues surrounding them. The roles of (1-3),(1-4)-beta-glucans in cell wall architecture and in cell growth are beginning to be understood. From biochemical experiments with active synthases in isolated Golgi membranes, the biochemical features and topology of synthesis are found to more closely parallel those of cellulose than those of all other noncellulosic beta-linked polysaccharides. The genes that encode part of the (1-3),(1-4)-beta-glucan synthases are likely to be among those of the CESA/CSL gene superfamily, but a distinct glycosyl transferase also appears to be integral in the synthetic machinery. Several genes involved in the hydrolysis of (1-3),(1-4)-beta-glucan have been cloned and sequenced, and the pattern of expression is starting to unveil their function in mobilization of beta-glucan reserve material and in cell growth.


Figures 2-4 are in color online.