Publication no. C-2002-1203-04R |  VIEW ARTICLE

Wheat Flour Proteins as Affected by Transglutaminase and Glucose Oxidase.

C. M. Rosell (1,2), J. Wang (1), S. Aja (1), S. Bean (3), and G. Lookhart (3,4). (1) Instituto de Agroquímica y Tecnología de Alimentos (CSIC). P.O. Box 73, 46100-Burjassot. Valencia. Spain. (2) Corresponding author. Phone: 34-96-390 0022. Fax: 34-96-363 6301. E-mail: <crosell@iata.csic.es> (3) Dept. Grain Science and Industry, Kansas State University, Manhattan, KS 66506. (4) USDA-ARS, Grain Marketing and Production Research Center, Manhattan, KS 66506. Cereal Chem. 80(1):52-55. Accepted August 21, 2002. Copyright 2003 American Association of Cereal Chemists, Inc.

Enzymes are good tool to modify wheat proteins by creating new bonds between the protein chains. In this study, the effect of the addition of glucose oxidase (GO) and transglutaminase (TG) on the wheat flour proteins is presented. The modification of wheat proteins was determined by analyzing the changes in gluten quality, alveograph parameters, and protein modifications. The amount of wet gluten increased with the addition of GO and TG, but the gluten quality was not improved in any case. Regarding the alveograph parameters, the effect of GO was readily evident obtaining wheat dough with higher tenacity and lower extensibility than the control, while TG led to doughs with lower tenacity and that were also less extensible. The protein modifications were characterized by free-zone capillary electrophoresis (FZCE). FZCE data indicated that TG polymerizes mainly glutenins and, of those, the high molecular weight glutenin subunits were the most affected.