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Publication no. C-2002-0401-06R
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ARTICLE
Corn Dry-Milled Grit and Flour Fractions Exhibit Differences in Amylopectin
Fine Structure and Gel Texture (1).
Y.-P. Lin (2), A. Aboubacar (2), B. E.
Zehr (3), and B. R. Hamaker (2,4). (1) Paper No. 15721 from Purdue Agricultural
Research Programs. (2) Department of Food Science and the Whistler Center for
Carbohydrate Research, Purdue University, West Lafayette, IN. (3) Mahyco, PO Box
27, Jalna, India 431 203. (4) Corresponding author. Department of Food Science,
1160 Food Science Building, Purdue University, West Lafayette, IN 47907-1160.
Fax: 765-494-7953. Cereal Chem. 79(3):354-358. Accepted December 3, 2001.
Copyright 2002 American Association of Cereal Chemists, Inc.
Corn starch amylopectin (AP) fine structure and gel textural properties of
dry-milled grit and flour fractions were investigated in 10 corn cultivars.
Amylopectin was isolated by fractionating the starch derived from these two
milled fractions using size-exclusion chromatography (SEC). Fine structure was
characterized by SEC after thorough debranching with pullulanase. SEC revealed
three major fractions of debranched AP from the grit and flour portion.
Amylopectin in the grit portion had a significantly higher proportion of long
chains (DP(n) 70-75) and a postulated lower extent of chain branching than its
flour counterpart. Texture profile analysis showed that flour gels from the grit
fraction had significantly higher values for hardness, gumminess, and
springiness compared with gels from the floury fraction. Trends were similar for
starch gels of the two dry-milled fractions, though only springiness was
significantly different. The finding that differences in AP fine structure in
dry-milled fractions relate to gel textural differences suggests that dry
millers may be able to produce flours of different functionalities that would be
suited for different end uses. Additionally, mixing the dry-milled flour
fraction with a grit-derived flour would result in different product properties.
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