Publication no. C-2001-1002-01R |  VIEW ARTICLE

Production of Boiling-Stable Granular Resistant Starch by Partial Acid Hydrolysis and Hydrothermal Treatments of High-Amylose Maize Starch.

Jorge O. Brumovsky (1) and Donald B. Thompson (1,2). (1) Department of Food Science, Penn State University, University Park, PA 16802. (2) Corresponding author: E-mail: <dbtl@psu.edu> Phone: 814-863-0481. Fax: 814-863-6132. Cereal Chem. 78(6):680-689. Accepted March 29, 2001. Copyright 2001 by the American Association of Cereal Chemists, Inc.

The purpose of the present work was to examine whether partial acid hydrolysis (PAH) of a high-amylose maize starch (ae-VII) would enhance the effects of hydrothermal treatments to produce granular resistant starch (RS) that is stable to further heat treatment at atmospheric pressure. PAH ae-VII starches were prepared by heating 35% (w/v) suspensions with 1% (w/w) HCl at 25°C for 6, 30, and 78 hr. Native and PAH starches were then treated by annealing (ANN) or heat-moisture treatment (HMT). ANN was done at 70% moisture at 50, 60, or 70°C for 24 hr, and HMT was done at 30% moisture at 100, 120, or 140°C for 80 min. RS that survives boiling during analysis was determined by a modification of the AOAC method for determining total dietary fiber. RS was also determined by the Englyst method. Little change in the gelatinization enthalpy was found for ae-VII starch after PAH, ANN, or HMT as individual treatments. After PAH, either ANN or HMT led to decreased gelatinization enthalpy. HMT and ANN alone increased boiling-stable RS but decreased total RS. After PAH of ae-VII, either ANN or HMT tended to increase the yield of boiling-stable granular RS, with the greatest yield (<63.2%) observed for HMT.