Publication no. C-2000-0417-01R |  VIEW ARTICLE

Laboratory Procedure to Wet-Mill 100 g of Grain Sorghum into Six Fractions (1).

X. J. Xie (2) and P. A. Seib (2,3). (1) Contribution 99-490-J from the Kansas Agricultural Experiment Station, Manhattan KS 66506. (2) Dept. of Grain Science and Industry, Kansas State University, Manhattan KS 66506. (3) Corresponding author: Fax: 785/532/7010. E-mail: <pas@wheat.ksu.edu> Cereal Chem. 77(3):392-395. Accepted February 10, 2000. Copyright 2000 American Association of Cereal Chemists, Inc.

A small-scale (100 g of grain) procedure was developed to wet-mill grain sorghum into six fractions by modifying the procedure of Eckhoff et al (1996). The wet-milling process was repeated five times on commercial grain sorghum, and the mean yield (69.4%) of starch (<=0.3% protein) varied by 0.3%, whereas the yields of fiber, gluten, and germ plus bran fractions varied by 5-6%. The starch fraction accounted for approximately 95% of that in the grain, while the total solids recovered was 99.0%. Four other samples of grain sorghum gave 92-95% recoveries of starches and 98.2-99.8% recoveries of total solids. All grain sorghum starches had lightness (L*) values and pasting curves nearly equal to those of a commercial maize starch.