Publication no. C-1998-0211-07R |  VIEW ARTICLE

Enzyme-Assisted Wet Separation of Starch from Other Seed Components of Hull-less Barley.

G.-H. Zheng (1) and R. S. Bhatty (2). (1) Department of Applied Microbiology and Food Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada. Phone: 306-966-5008. Fax: 306-966-8898. E-mail: <zhengg@skyfox.usask.ca> (2) Crop Development Center, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada. Cereal Chem. 75(2):247-250. Accepted December 12, 1997. Copyright 1998 by the American Association of Cereal Chemists, Inc.

A multiple enzyme cocktail containing cellulase, endo-(1-3),(1-4)-beta-D-glucanase and xylanase was used in wet separation of starch, protein, beta-glucan, bran, and tailings from four hull-less barleys (HB): SB94794 (0% amylose), CDC Candle (5% amylose), CDC Dawn (24% amylose), and SB550831 (40% amylose). Compared to a conventional procedure, the enzyme-assisted wet extraction reduced slurry viscosity by 50-99%, the amount of water and ethanol used in screening and beta-glucan precipitation by 30-60%, and screening time by 20-80%. The enzyme-assisted extraction reduced starch contents and yields of tailings and bran fractions, resulting in a 10% increase in average starch extraction efficiency. However, beta-glucan yield was reduced in the enzyme-assisted extraction, particularly in high-viscosity HB. The physicochemical properties of isolated starches were not affected by the enzyme-assisted extraction.