Publication no. C-1998-0108-02R |  VIEW ARTICLE

Fusarium Species and 8-Keto-Trichothecene Mycotoxins in Manitoba Barley (1).

D. Abramson (2,3), R. M. Clear (4), E. Usleber (5), R. Gessler (5), T. W. Nowicki (4), and E. Märtlbauer (5). (1) Contribution 1700 of the Cereal Research Center, and 756 of the Grain Research Laboratory, Winnipeg, Canada. (2) Cereal Research Center, Agriculture and Agri-Food Canada, Winnipeg R3T 2M9, Canada. (3) Corresponding author. E-mail: <dabramson@em.agr.ca> (4) Grain Research Laboratory, Canadian Grain Commission, Winnipeg R3C 3G8, Canada. (5) Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, Germany. Cereal Chem. 75(1):137-141. Accepted September 14, 1997. Copyright 1998 Department of Agriculture and Agri-Food, Government of Canada.

Fusarium head blight, induced primarily by Fusarium graminearum, resulted in widespread damage to the Manitoba barley crops of 1993 and 1994, with contamination by deoxynivalenol (DON) and other 8-keto-trichothecenes. Visible Fusarium mold in samples of 1994 barley had little relationship to DON levels in the kernel as determined by gas chromatography-mass spectrometry (GC-MS). While samples of 1993 and 1994 barley showed a weak correlation between the logarithm of DON level and percentage of kernels infected by Fusarium graminearum (r = 0.79 and 0.71, respectively), the latter method is too lengthy and requires too much training for commercial application. A commercial enzyme immunoassay for DON gave results that correlated well with GC-MS methods (r = 0.95 and 0.89, respectively) in samples of 1993 and 1994 barley and afforded a rapid and convenient method for screening. In barley samples from 1994, DON, 15-acetylDON, 3-acetylDON and 3,15-diacetylDON were detected in the approximate ratio of 47:4:1:1. In view of the higher oral toxicities of 15-acetylDON and 3-acetylDON relative to DON, and the unknown oral toxicity of 3,15-diacetylDON, GC-MS assays might be advisable in samples positive for DON from enzyme immunoassay screening.